Table 2.
Primers used for DNA Methylation Analysis.
| Genome | Accession Number | Forward Primer Sequence | Reverse Primer Sequence | Tm (°C) | Size (bp) |
|---|---|---|---|---|---|
| SOD1 | NT_176367.1 | CATCCATCTTGATGGGTCCT | GCGCCGGACTCGATTTA | 57 | 222 |
| SOD2 | NT_176359.1 | GGATCCCTGGGGTGATGTT | GCCCCATAGTCGTAGGGTAA | 58 | 172 |
| SOD3 | NT_176369.1 | GGATGAGGTGGATCTGTCG | GGCATACATGTCTCCGATCT | 57 | 227 |
| GPx1 | NT_176411.1 | CGAGAAGTTGGGGAGGAGTA | CGGACGTACTTGAGCGAATG | 57 | 241 |
| GPx3 | NT_176338.1 | TTCCACCAAAAGTGGGGGTC | GGCCTCAAGCAAGTCTTGGA | 60 | 183 |
| GPx4 | NT_176099.1 | ACCCCTGGCTTGATCGGAAC | CAGCTTCCAGGCACGGTCA | 61 | 153 |
| CAT | NT_176327.1 | GCAGAGTTTGAAGTCGCCTA | ACCTAAAACGCCACACAAAC | 57 | 310 |
Sequences are given from 5′ → 3′. The optimal melting temperature (Tm) is indicated for each primer pair. Expected lengths of the PCR products are shown in base pairs (bp).