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. Author manuscript; available in PMC: 2016 Jul 22.
Published in final edited form as: Cell Death Discov. 2016 Jul 4;2:16045. doi: 10.1038/cddiscovery.2016.45

Figure 4.

Figure 4

LPO is correlated with the extent of AS induced by IR and topoisomerase inhibitors. (a) Imaging of LPO in living cells using C11-BODIPY probe. B16-F10 cells were treated with either dimethyl sulfoxide (DMSO) vehicle (0.5%) or etoposide (2 μM) for 96 h to induce senescence. Cells were imaged at × 20 magnification (upper row) using identical settings. An enlarged section of each image is shown (lower row) to demonstrate cellular distribution of the oxidized probe. Etoposide induces LPO and the oxidized lipid probe appears to accumulate in organelles, lipid droplets and the perinuclear region. (b and c) LPO induced by (b) IR and (c) topoisomerase inhibitors. Cells were treated, stained with C11-BODIPY and analyzed by flow cytometry. MFI for each sample condition is shown. Samples included 10 000 cells. (d and e) Strong correlation of LPO with SI for cells treated with (d) IR and (e) topoisomerase inhibitors. These results provide evidence that a combination of DNA damage and LPO may synergize to drive AS.