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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Cell Mol Bioeng. 2016 Jul 6;10(1):30–40. doi: 10.1007/s12195-016-0456-5

FIGURE 1.

FIGURE 1

Quantification of cholesterol enrichment using cholesterol-cyclodextrin complexes. Cells were incubated with either PBS with calcium/magnesium or 3.5 mM cholesterol for 30 min at 37 °C. Cells were lysed, and protein and cholesterol content were assayed. Cholesterol enriched cells showed a 3.5× increase in cholesterol as compared to unenriched cells. (Means and SEM were plotted, two-tailed t test ***p<0.0001 n = 6 unenriched, n = 6 cholesterol).