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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Cell Mol Bioeng. 2016 Jul 6;10(1):30–40. doi: 10.1007/s12195-016-0456-5

FIGURE 2.

FIGURE 2

Cholesterol inhibits ATP-induced eNOS phosphorylation. Cells were simulated with 100 μM ATP with Ca+2. Cells were treated with either PBS with Ca+2 or 3.5 mM cholesterol at 37 °C for 30 min prior to stimulation. Cells were harvested before stimulation (t = 0) and at time points 1, 3, 5, and 10 min after stimulation. All p-eNOS/eNOS ratios were normalized by t = 0. eNOS phosphorylation in cholesterol enriched cells (Cholesterol) is abolished in response to ATP. (Mean and SEM were plotted p <0.05*, #; p <0.01** two-tailed t test; Unenriched n = 4, Cholesterol n = 4).