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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: JCI Insight. 2016 Aug 4;1(12):e87315. doi: 10.1172/jci.insight.87315

Figure 1. Intravital 2-photon imaging reveals impaired neutrophil tra_cking in heart grafts that lack monocyte-derived macrophages.

Figure 1

(A) Control PBS liposome–treated heart graft with neutrophil (green) arrest inside blood vessels (blood vessels appear red after injection of quantum dots), intravascular cluster formation, and extravasation (see Supplemental Video 1; n = 4 mice). Neutrophil tra_cking in hearts derived from (B) donors that received treatment with clodronate liposomes prior to organ harvest (see Supplemental Video 2; n = 4 mice). Relative time is displayed in hrs:min:sec. Scale bars: 50 μm. (C) Percentage of neutrophils that extravasated during imaging period was significantly higher in hearts derived from control PBS liposome–treated donors compared with heart grafts harvested from clodronate liposome–treated mice. (D) Neutrophil rolling velocities were comparable in coronary veins of cardiac grafts derived from control PBS liposome–treated and clodronate liposome–treated mice. (E) Intraluminal crawling velocities were significantly lower in hearts harvested from clodronate liposome–treated WT compared with PBS liposome–treated mice. *P < 0.05; **P < 0.01 (t test). Data in C, D, and E are derived from 4 mice for each experimental group. For D and E, symbols represent averages obtained from individual mice with over 30 neutrophils examined per mouse, horizontal bars denote means, and error bars denote ±SEM.