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. Author manuscript; available in PMC: 2016 Nov 7.
Published in final edited form as: J Pediatr Gastroenterol Nutr. 2016 Nov;63(5):524–530. doi: 10.1097/MPG.0000000000001375

Figure 1. Expression of add3a and xpnpep1 in the developing liver.

Figure 1

Whole-mount in-situ hybridization (ISH) of 3 days post fertilization (dpf) zebrafish larvae probed for add1 (A), add2 (B), add3a (C), add3b (D) and xpnpep1 (E). There is liver staining for add3a (C, black arrow) and xpnpep1 (E, black arrow) but not for the other probes. There is intestinal staining for both add3a, add3b and xpnpep1 (black arrowheads in C, D, E). There is also staining of add2 and add3a in the head region, which is of unclear specificity (B, C). Similar experiments were performed using a riboprobe directed against xpnpep2, but there was no staining in 3 dpf larvae (not shown).