Spontaneous pain behaviors (A) guarding and (C) flinching were recorded in a two-minute period 14 days post-inoculation and at various time points after drug administration. Time of peak effect at 15 minutes post-administration (p < 0.01 for 0.36 and 1 μg/kg, compared to saline) lasting for two hours. (B) Emax= 52.75%, A90= 0.058 μg/kg for the guarding curve, (D) A90= 1 μg/kg for the flinching curve. Tactile allodynia was measured 14 days post-inoculation and at various time points after drug administration; a significant (p < 0.05, n=8) reduction in evoked pain behaviors with an onset 30 min was measured after injection of 0.360 and 1 μg/kg and persisted for nearly 2 hours (E). Area under the curve was calculated (F). Values represent the mean ± standard error of the mean, n=8 per group.