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. Author manuscript; available in PMC: 2016 Nov 23.
Published in final edited form as: Pain. 2016 Dec;157(12):2709–2721. doi: 10.1097/j.pain.0000000000000690

Figure 5. MasR is expressed in the dorsal root ganglion and femur extrudate.

Figure 5

(A) Ipsilateral DRGs were harvested from naïve, sham (media inoculated) or chronically-treated 66.1 inoculated mice, homogenized, and probed for MasR expression via Western blotting. MasR is expressed in the dorsal root ganglia; MasR bands were observed at ~50 kDa and ~40 kDa (B). Sham surgery (i.e. media only) did not alter MasR expression levels in the DRGs. 66.1-inoculation increased the expression of MasR in the ipsilateral DRGs, though not statistically significant. β-actin serves as the loading control. (Pools of DRGs from n=3–5 animals) (C) Ipsilateral bone marrow extrudate was harvested from naïve, sham (media-inoculated), or cancer-inoculated mice and probed for MasR expression via Western blotting. MasR is expressed in the femur extrudates of mice. MasR bands were observed at ~50 kDa and ~40 kDa. (D) Sham surgery (i.e., media only) did not alter MasR expression levels at ~40 kDa or ~50 kDa in the femur extrudate. 66.1-inoculation significantly increased (p < 0.001) the expression of MasR in the ipsilateral femur extrudate. β-actin serves as the loading control. (Pools of femur extrudate from n=3–5 mice)