Skip to main content
. Author manuscript; available in PMC: 2017 Apr 6.
Published in final edited form as: J Magn Reson Imaging. 2016 Sep 30;45(4):1144–1153. doi: 10.1002/jmri.25459

Figure 4. Histological analysis of carotid arteries and correlation with T2* signal loss on MRI.

Figure 4

Figure 4

(A) Immunohistochemical staining of the ligated left carotid lesions showed substantial macrophage infiltration of the neointima (two-headed arrows) and peri-adventitia (arrows). Perl’s iron staining showed greater accumulation of RGD-HFn-Fe3O4 in the neointima (ligated carotid RGD+) compared to HFn-Fe3O4 (ligated carotid RGD−), colocalizing with macrophages (outline region). Immunohistochemical staining also showed positive endothelial cell staining along the luminal border, but few endothelial cells in the neointima and adventitia. The control right carotid arteries showed minimal Perl’s iron staining in both RGD+ and RGD− groups. (B) There was a close correlation between the total number of Perl’s iron stained cells and % lumen loss in the ligated left carotid artery (n = 10, r = 0.92, p = 0.0002).