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. Author manuscript; available in PMC: 2016 Sep 16.
Published in final edited form as: JCI Insight. 2016 Sep 8;1(14):e88468. doi: 10.1172/jci.insight.88468

Figure 5. HE-directed mutagenesis of latent HSV in vivo.

Figure 5

(A) Levels of AAV genomes were quantified by ddPCR in right (ipsilateral) TGs from infected and naive mice. Mean ±SD are indicated. (B) Mutagenic event detection by T7E1 assay. The HSV regions containing the target site for HSV1m5 (top panel) or HSV1m8 (bottom panel) were PCR amplified from total genomic DNA obtained from the right (ipsilateral) TG. Products were subjected to T7E1 digestion and separated on a 3% (HSV1m5) or 1% (HSV1m8) agarose gel. mw, molecular weight size ladder. (C) Levels of latent HSV genomes were quantified by ddPCR in right (ipsilateral) TGs from infected and naive mice. Mean ±SD are indicated.