Table 1.
Fold change in mRNA expression of genes associated with metal transport, detoxyfication and antioxidant response in skn-1(ok2315) worms 24 hours post MeHg exposure
| MeHgCl | 0 μM | 5 μM | 25 μM | 50 μM | ||||
|---|---|---|---|---|---|---|---|---|
| GEE | 0 (μg/ml) | 1000 (μg/ml) | 0 (μg/ml) | 1000 (μg/ml) | 0 (μg/ml) | 1000 (μg/ml) | 0 (μg/ml) | 1000 (μg/ml) |
| Gene | ||||||||
| mtl-1 | +0.01e-2 (0.04) | +0.24 (0.13) | −0.15 (0.33) | +0.07 (0.31) | +1.16 (0.79) | +1.91 $ (0.60) | +0.32 (0.56) | +5.25 *$ (0.69) |
| mtl-2 | +0.06e-8 (0.03) | +0.31 (0.17) | −0.14 (0.20) | +1.36 $* (0.37) | −0.26 (0.10) | +0.01 (0.30) | −0.58 (0.08) | +0.08 (0.16) |
| aat-2 | +0.09e-8 (0.04) | +0.01 (0.14) | +0.26 (0.15) | +1.6 $* (0.47) | −0.29 (0.09) | +0.31 (0.17) | −0.27 (0.08) | −0.30 (0.04) |
| sod-3 | −0.01e-2 (0.03) | +0.24 (0.14) | −0.35 (0.04) | +1.11 $* (0.24) | −0.22 (0.08) | +0.48 * (0.26) | −0.86 # (0.02) | −0.63 $ (0.08) |
| sir-2.1 | +0.52e-9 (0.05) | +0.26 (0.08) | +0.02 (0.06) | +1.26 $* (0.21) | −0.10 (0.09) | +0.35* (0.01) | −0.27 (0.06) | −0.03 (0.08) |
#
means significant difference between worms exposed and non-exposed to MeHgCl,
$
means significant difference between GEE pre treated worms exposed and non exposed to MeHgCl and
*
means significant difference between GEE pre treated and untreated worms by two-way ANOVA followed by Bonferroni’s Multiple Comparison test (p<0.05)
This experiment was assessed by RT-qPCR. and carried out in three independent worm preparations, each in triplicate. Data are expressed as means ±SEM in parentheses.