Skip to main content
. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Pharmacol Exp Ther. 2008 Dec 9;328(3):715–722. doi: 10.1124/jpet.108.147330

Figure 4. Disorazole C1 disrupts microtubules at interphase and metaphase.

Figure 4

Mammalian PtK2 cells were treated with the indicated concentrations of either O1OA (inactive analog of disorazole C1) or disorazole C1 for 24 h at 37°C. Cells were fixed and stained with anti-α-tubulin antibody to visualize microtubules (red) and Hoechst 33342 to visualize chromosomes (blue). All images were collected with a Nikon E600 microscope equipped with a Roper CoolSnap HQ digital camera at 40X (interphase) or 100X objectives (mitotic spindles). Upper panels, interphase; lower panels, mitosis. Bars = 10 μm.