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. Author manuscript; available in PMC: 2018 Jan 15.
Published in final edited form as: Biosens Bioelectron. 2016 Sep 2;87:646–653. doi: 10.1016/j.bios.2016.09.006

Fig. 2. Effect of different buffers on DNA hybridization.

Fig. 2

(A) SYBR Green fluorescence in the presence of complementary (solid line) and non-complementary (dashed line) targets incubated with 24-base ssDNA probe in 1 M 1×TE-NaCl buffer (black), 100 mm 1×TE-NaCl buffer (red), 1mM 1×TE-MgSO4 buffer (blue) and 100 μM 1×TE-MgSO4 buffer (green) within temperature range from 4 °C to 95 °C. (B) Corresponding relative fluorescence change between the complementary and non-complementary targets in four different buffers. The black dashed box indicates the temperature used in all the experiments.

RFU = Relative fluorescence units