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. Author manuscript; available in PMC: 2009 Jun 3.
Published in final edited form as: J Neurosci. 2008 Dec 3;28(49):13223–13231. doi: 10.1523/JNEUROSCI.2814-08.2008

Figure 5.

Figure 5

a. Confocal images showing the distribution of AChR clusters in wild type embryos visualized by α-Btx (left), and the distribution of AChR clusters visualized by YFP in δ2YFP/sop−/− embryo, stably expressing δ2YFP (right). Both images were obtained at 3dpf.

b. mEPCs recorded from a single slow-twitch muscle cell of a wild type embryo (left) and a δ2YFP/sop−/− embryo (right) at 3dpf. Amplitude and decay time constant of individual mEPCs are plotted. Averaged traces are shown as insets. Scale: 50 pA, 6 ms.

c. mEPCs recorded from a single fast-twitch muscle cell of a wild type embryo (left) or a δ2YFP/sop−/− embryo (right). Amplitude and decay time constant of individual mEPCs are plotted. Averaged traces are shown as insets. Scale: 50 pA, 6 ms.

d. EPC recordings in response to spinal cord stimulation. Stimulation artifacts are marked with asterisks. Scale: 500 pA (wild type), 200 pA (δ2YFP/sop−/−), 5 ms.

e. Accumulated data of fast-twitching and slow-twitching muscle cells from wild type and δ2YFP/sop−/− embryos. Current amplitude (left), rise time (middle) and decay time constant (right) of mEPCs are shown. Bar graphs represent the mean and error bars are SEM.