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. Author manuscript; available in PMC: 2010 Mar 4.
Published in final edited form as: J Neurosci. 2009 Mar 4;29(9):2948–2960. doi: 10.1523/JNEUROSCI.4424-08.2009

Figure 2. PSD-95 inhibits D1-NR1 interaction.

Figure 2

(A) Coimmunoprecipitation of HA-D1 and NR1 in the presence or absence of PSD-95 overexpression in HEK293T cells. (B, C) Densitometric analyses of total (B) and coprecipitated (C) NR1 and D1 receptors. n = 3–4; data represent mean ± s.e.m. *, P < 0.05, two-tailed Student’s t test. (D) Schematic drawing of PSD-95 truncation constructs. (E) Effects of PSD-95 mutants on D1-NR1 interaction, as measured by coimmunoprecipitation in the presence or absence of overexpression of different PSD-95 constructs. HEK293T cells were transfected with HA-D1, NR1/NR2B, and different PSD-95-GFP constructs or a mock vector. Coimmunoprecipitation was performed by incubating HEK293T cell lysates with an anti-NR1 antibody followed by immunoblotting with an anti-HA or an anti-NR1 antibody. Total levels of D1 and NR1 were measured by western blots. Experiments were repeated 4 times with similar results.