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. Author manuscript; available in PMC: 2017 Apr 6.
Published in final edited form as: Anat Rec (Hoboken). 2017 Jan 11;300(3):560–576. doi: 10.1002/ar.23511

Figure 3.

Figure 3

Representative confocal micrographs of FVB and JTMT renal cortex frozen sections immunofluorescently stained for MT and EC. A. FVB: MT expression visualized with FITC (green) immunofluorescence. There was little to no MT expression in tissue sections from the control animal. B. JTMT: MT expression visualized with FITC (green) immunofluorescence. In the transgenic mouse, strong MT staining was observed in the glomerulus (blue arrow), peritubular capillaries (orange arrow) and arterioles (yellow arrow). C. FVB: Merged tomato lectin (red) and MT (green) immunofluorescent staining. The control mouse displayed limited MT expression and it was not associated with the vasculature. D. JTMT: Merged tomato lectin (red) and MT (green) immunofluorescent staining. Due to the somewhat “leakiness” of the TIE −2 promoter (tends to promote staining of hematopoietic cells) and its ability to bind non-vascular structures in the kidney, there was limited co-distribution (yellow staining) of MT with the tomato lectin. However, the MT overexpression was localized to EC in the glomerulus (blue arrow), peritubular capillaries (orange arrow), and arterioles (yellow arrow).