Skip to main content
. Author manuscript; available in PMC: 2017 Jul 25.
Published in final edited form as: J Vis Exp. 2017 Feb 16;(120):10.3791/55382. doi: 10.3791/55382

Figure 2. Representative cytograms demonstrating enrichment of Tet Tox-binding B cells from human peripheral blood.

Figure 2

(A) Cytograms of enriched and depleted Tet Tox-reactive B cells from Fraction A and total Tet-Tox-reactive B cells from Fraction D from a normal subject last vaccinated >10 years previously. (B) Cytograms of PBMCs from the “positive” populations from a subject vaccinated ~3 years previously and enriched as in A (Fraction A), after pre-incubation with excess of 50 uM unlabeled Tet-tox (Fraction B), or enriched with Tet-Tox-biotin omitted during the procedure (Fraction C). All analyses were gated on CD19+ lymphocytes. Percentages represent the percent of Tet-Tox-binding cells of all B cells (CD19+) in the final fraction.