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. Author manuscript; available in PMC: 2017 May 15.
Published in final edited form as: Chem Sci. 2016 Nov 25;8(3):2107–2114. doi: 10.1039/c6sc02297a

Fig. 2.

Fig. 2

Evaluation of the CuAAC reactivity of ligands 1–3 using a fluorogenic CuAAC reaction. Reaction conditions: 100 µM 5, 50 µM 6, 100 µM CuSO4, 200 µM ligand, 500 µM sodium ascorbate (NaAsc) in 10/90 (v/v) methionine-free DMEM/PBS, room temperature. Yield was derived from the measured mean fluorescence intensity of 7 (Fig. S4†). Controls were performed without HPG. Error bars represent the standard deviation of data from three samples.