(A) WT mice were passively immunized with either a control IgG or anti-IL-36γ IgG and infected with i.t. Sp. Total BAL leukocytes were quantified, and macrophage and neutrophil counts were assessed by manual differential (No significant differences between groups at the same time point by one-way ANOVA with Sidak’s multiple comparisons test, n = 5 mice/group, representative of 2 experiments). (B) WT and IL-36γ−/− were infected with i.t. Sp. Total leukocytes, macrophages, and neutrophils were assessed as above (No significant differences between groups at the same time point by one-way ANOVA with Sidak’s multiple comparisons test, n = 4–6 mice/group, representative of 3 experiments). (C) WT and IL-36γ−/− were infected with i.t. Sp. Lungs were homogenized at the specified time points and otal leukocytes, macrophages, and neutrophils were assessed by manual differential (No significant differences between groups at the same time point by one-way ANOVA with Sidak’s multiple comparisons test, n=3 mice per group per time point).