Figure 4. Particle adhesion to inflamed mesentery endothelium as a function of varied ratios of sLe^A and aICAM sites.

(A) Diagram of five particle conditions with varied amounts of sLe^A and aICAM ligand density at a constant total site density of 10,000 sites/µm². (B) Representative fluorescence image of particle I (7,500 sites/µm² sLe^A: 2,500 sites/µm² anti-ICAM) adhesion to inflamed mesentery, scale bar 50 µm. (C) Quantified number of fixed or rolling particles on inflamed mesentery per representative imaging segment, n=3 mice. Statistical analysis of adherent density was performed using one-way ANOVA with Fisher’s LSD test between total adherent particles: (*) indicates p<0.05 and two-way ANOVA with Fishers LSD test between groups (##) p<0.01 between firm groups, rolling groups n.s. (D) Velocity of rolling particles found at mesentery wall, n ≥ 50 particles from n = 3 mice. Statistical analysis of rolling velocity was performed using one-way ANOVA with Fisher’s LSD test, (*) indicates p<0.05, n.d. indicates none detected and excluded from the analysis. Error bars represent standard error.