Skip to main content
. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Allergy. 2017 Jan 23;72(6):866–887. doi: 10.1111/all.13119

Figure 3. Examples of expression systems used for recombinant expression of anti-tumour IgE.

Figure 3

A. Recombinant IgE by cloning the variable domains of IgG of desired specificity to an IgE constant domain. B. Classical restriction enzyme-based cloning requires the presence of specific restriction sites flanking the gene of interest. C. Expression of IgE by insect cells requires a recombinant baculovirus stock containing the antibody expression cassette. D. Polymerase Incomplete Primer Extension (PIPE) cloning facilitates a rapid cloning of DNA sequences with the option of performing site-specific mutagenesis at the same time.