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. Author manuscript; available in PMC: 2017 Jan 11.
Published in final edited form as: J Vis Exp. 2016 Nov 27;(117):10.3791/53992. doi: 10.3791/53992

Figure 3. Evidence for widespread physiological caspase activity.

Figure 3

(a) Merged confocal image of CaspaseTracker (DQVD) RFP and nucGFP from a single newly eclosed fly raised at 18 °C, dissected and stained with H33342 for nuclei and Phalloidin for F-actin (pink), and imaged with DIC. Phalloidin is shown only in the inset for egg chambers. (b) The same procedure and imaging conditions were followed for control CaspaseTracker (DQVA) flies. *Autofluorescent structures. These data are reproduced with permission from Tang et al., Sci Rep 2015.