Biochemical analysis of tau in Triton X-100 insoluble fraction extracted from frozen brain tissues. (A) Total-tau (17025) and (B) phosphorylated-tau (PHF-1) immunoblot showed abundance of tau pathology in Case-1 and Case-2 Hp, but not in Case-1 Pu. Isoform specific immunoblot revealed predominant 4R-tau pathology in both cases (C) consistent with IHC finding. Further, Case-1 Hp was also immune-reactive to 3R-tau antibody (d). Hp = hippocampus, Pu = putamen; Alzheimer disease (AD), corticobasal degeneration (CBD), and Pick`s disease (PiD) control samples are also shown.