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. Author manuscript; available in PMC: 2017 Mar 20.
Published in final edited form as: Eur J Immunol. 2017 Jan 25;47(3):458–469. doi: 10.1002/eji.201646855

Figure 2.

Figure 2

AQP4(201–220) is the immunodominant IAb-restricted epitope of AQP4. WT C57BL/6 and Aqp4−/− mice were immunized s.c. with full-length AQP4 protein (A, B, and D) or AQP4(201–220) peptide (C) emulsified in CFA. Draining LN cells and splenocytes were analyzed for cell proliferation in response to single peptides or full-length AQP4 protein. (A, B) Splenocytes and LN cells from AQP4 immunized WT and Aqp4−/− mice were stimulated with single 20-mer peptides spanning the whole sequence of AQP4. (C) Draining LN cells of AQP4(201–220) immunized WT and Aqp4−/− mice were tested for their proliferative recall response to full-length AQP4 protein. (D) Fine mapping of the immunodominant AQP4 epitope was performed using 11-mer peptides spanning AQP4(196–225) to recall cells from AQP4-immunized Aqp4−/−mice. Proliferation was measured by 3[H]-thymidine incorporation. Means of triplicate cultures ± SD are shown. **p < 0.01, ***p < 0.001 (Student’s t-test). Data are representative of five independent experiments (A–D).