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. Author manuscript; available in PMC: 2017 Oct 25.
Published in final edited form as: Arthritis Rheumatol. 2017 Apr 28;69(6):1246–1256. doi: 10.1002/art.40080

Figure 5. Blocking of LRP1 sheddases restores endocytic capacity and reduces the degradation of aggrecan and collagen in OA cartilage.

Figure 5

(A) Recovery of endocytic capacity in OA chondrocytes upon treatment with anti-ADAM17 and anti-MMP-14 antibodies. Endocytosis of ADAMTS-5 (10 nM) was measured as in Figure 1 (H and I). Data are expressed as the mean ± SD (n=3). (B and C) Inhibition of aggrecan and collagen degradation in human OA cartilage upon treatment with anti-ADAM17 and anti-MMP-14 antibodies, respectively. OA knee cartilage explants were incubated with antibodies or TIMPs at the concentration of 250 nM. (B) Aggrecan degradation detected after 48 h by an aggrecanase-cleavage specific anti-374ARGS neoepitope antibody. The mean value in medium of the cartilage incubated with the control antibodies was taken as 100 (n=8). (C) Collagen degradation of cultured OA cartilage after 96 h incubation was measured by hydroxyproline assay. The amount of hydroxyproline released with control antibodies was taken as 100. Each point represents an individual cartilage donor (mean ±SD). *, p < 0.05, by 2-tailed Student’s t test (A) or one-way ANOVA followed by Dunnett’s multiple comparison test (C).