Table 1.
PCR setup for amplification of the RGR unit
| DNA template | 20 ng |
| dNTPs (2 mM) | 10 μl |
| Forward primer (20 μM) | 2.5 μl |
| Reverse primer (20 μM) | 2.5 μl |
| 5x PCR buffer | 20 μl |
| Phusion DNA polymerase (NEB) | 1 μl |
| Add nuclease free H2O to 100 μl |
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