Table 3.
In vitro DNA assembly
| Final PCR product and the vector | 0.03–0.2 pmols, X μl |
| Recommended DNA ratio | Vector:Insert = 1:2 |
| NEB HiFi DNA assembly master mix (Gibson Assembly® Cloning Kit) | 10 μl |
| Water | 10-X μl |
| Total Volume | 20 μl |
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