Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
In vitro transcription of RGR to produce gRNA molecules
| Total | 20 μl |
| 5x transcription buffer | 4 μl |
| 100 mM DTT | 2 μl |
| ~20 U/μl recombinant RNasin RNase inhibitor | 0.5 μl |
| 2.5 mM each rNTPs | 4 μl |
| Template DNA (PCR fragment) (10–50 ng/μl [final]) | x μl |
| T7, SP6 or T3 RNA polymerase | 1 μl |
| H2O | (8.5 - x) μl |
