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. Author manuscript; available in PMC: 2017 Aug 5.
Published in final edited form as: Bio Protoc. 2017 Feb 5;7(3):e2119. doi: 10.21769/BioProtoc.2119

Figure 1. FACS setup for isolation of single GFP positive cells.

Figure 1

GFP positive cells are isolated by first selecting viable cells (A), then removing doublets (B), then identifying all GFP positive cells (C) and finally selecting the top 10% of GFP expressing cells for sorting (D). Gating hierarchy is shown in panel E.