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Composition of Differentiation Medias.
| Name | Composition | Differentiation days used |
|---|---|---|
| Differentiation Media I | RPMI 1640 B27 Supplement (minus insulin) Penicillin-Streptomycin |
D0-D1 (with CHIR99021) D2 D3-D5 (with IWR-1) D5-D7 |
| Differentiation Media II | RPMI 1640 B27 Supplement (with insulin) Penicillin-Streptomycin |
D7-D20 |
