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. Author manuscript; available in PMC: 2009 Mar 20.
Published in final edited form as: J Proteome Res. 2008 Aug 13;7(9):3900–3910. doi: 10.1021/pr800198w

Figure 1.

Figure 1

(A) Schematic for the integrated proteomic approach for the identification of c-Src kinase substrates. Human embryonic kidney (HEK) 293T cells growing in Arg `0' containing medium were transiently transfected with a kinase-dead Src (K298M) and 293T cells growing in Arg `6' and Arg `10' were transiently transfected with constitutively active Src kinase (Y527F). Arg `0' refers to 12C6-arginine, Arg `6' refers to 13C6-arginine and Arg `10' refers to 13C6-15N4-arginine, isotopic labeled forms of arginine used to differentially label 293T cells for identification of Src substrates.