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. Author manuscript; available in PMC: 2017 Oct 20.
Published in final edited form as: JACC Basic Transl Sci. 2017 Apr 24;2(2):160–180. doi: 10.1016/j.jacbts.2016.12.002

Figure 4. Specificity of PP2Ce activity to PLN Thr-17 phosphorylation.

Figure 4

A. Immunoblot of PP2Ce, GFP, and PLN phosphorylation (Ser-16 and Thr-17) in NRVM expressing GFP or PP2Ce wildtype or PP2Ce phosphatase dead mutant (D320A) following 1μM isoproterenol treatment at different time points as indicated. B. ISO induced PP2Ce expression and PLN phosphorylation in NRVM expressing PP2Ce truncation mutant (Δ1-57) which has lost ER targeting capacity ((28, 29)). C. PP2Ce–GST fusion protein was incubated with PLN isolated from NRVM with or without ISO treatment, followed by immunoblot for total and phosphorylation levels of PLN at Ser-16 and Thr-17. D. Co-immunoprecipitation of PP2Ce and PLN in mouse heart using PP2Ce TG and PP2Ce KO as positive and negative control, respectively.