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. Author manuscript; available in PMC: 2017 Oct 20.

Published in final edited form as: JACC Basic Transl Sci. 2017 Apr 24;2(2):160–180. doi: 10.1016/j.jacbts.2016.12.002

A. Schematic drawing of PP2Ce-transgenic mouse with tissue specific expression of PP2Ce in heart. B. PP2Ce protein expression in mouse hearts from four genotypes carrying either none, single or double transgenes of αMHC-tTA expressing tet-off suppressor (tTA) and αHMC-TetO-PP2Ce (iPP2Ce). PP2Ce expression was detected only from double transgenic hearts in the absence of tetracycline. PC is positive control from NRVM expressing adv-PP2Ce. C. Representative immunot blot analysis. D. Quantification of key proteins involving in calcium homeostasis regulation in control and PP2Ce transgenic (TG) hearts. Number of replicates is labeled in each column as indicated. *: p<0.05, **: p<0.01. E. Calcium transient peak amplitudes (F/F0), time to peak and decay measured from isolated cardiomyocytes of control (n=8) and PP2Ce TG (n=8) hearts.

A. Schematic drawing of PP2Ce-transgenic mouse with tissue specific expression of PP2Ce in heart. B. PP2Ce protein expression in mouse hearts from four genotypes carrying either none, single or double transgenes of αMHC-tTA expressing tet-off suppressor (tTA) and αHMC-TetO-PP2Ce (iPP2Ce). PP2Ce expression was detected only from double transgenic hearts in the absence of tetracycline. PC is positive control from NRVM expressing adv-PP2Ce. C. Representative immunot blot analysis. D. Quantification of key proteins involving in calcium homeostasis regulation in control and PP2Ce transgenic (TG) hearts. Number of replicates is labeled in each column as indicated. *: p<0.05, **: p<0.01. E. Calcium transient peak amplitudes (F/F0), time to peak and decay measured from isolated cardiomyocytes of control (n=8) and PP2Ce TG (n=8) hearts.