Skip to main content
. Author manuscript; available in PMC: 2017 Jun 2.
Published in final edited form as: J Vis Exp. 2016 Nov 8;(117):10.3791/54720. doi: 10.3791/54720

Figure 2. Functional Analyses of In Vitro Differentiated Ag-specific Tregs.

Figure 2

Murine iPSC-Tregs were stimulated by splenocytes (APCs; Tregs/APCs = 1:4) and pulsed with OVA323-339 peptide. Intracellular cytokine production (TGF-β and IL-10) was analyzed by flow cytometry after gating on live CD4+ CD25+ cells (dark lines; shaded areas indicate isotype controls).