Figure 6. In vivo.
monitoring of PEG17-PPS28-PBI-PPS28-PEG17 polymersome disassembly within lymph node resident immune cell populations. Polymersomes (10 mg/mL) or PBS controls (ctr) were injected subcutaneously into mice and brachial lymph nodes were extracted at the indicated time points for analysis by flow cytometry. Histograms show the average percentages ± standard deviation of polymersome positive (PS+) cells for each indicated cell type. Macrophages: CD11b+F4/80+; dendritic cells (DCs): CD11c+; mature DCs (mDCs): I-A/I-E+CD11c+; plasmacytoid DCs (pDCs): I-A/I-E+CD11c+Gr-1+B220+; B cells: CD45+CD19+; natural killer (NK) cells: CD45+CD49b+; granulocytes: Gr-1+CD11b+. Statistical significance: *p ≤ 0.1, **p ≤ 0.05, ***p ≤ 0.001.