A. Expression of MRP2, a multidrug transporter responsible for clearance of bilirubin is downregulated in LKO and LPKO livers. Numbers below the gel image are densitometry quantification of the image shown. B. MRP2 is redistributed from the apical membrane (arrows) in the CON and PKO livers to a diffused cytoplasm distribution in the LKO and LPKO livers. Scale bar, 100µm. C. Liver function index. Left, plasma bilirubin levels is also increased in LKO and LPKO livers at 3 weeks of age. Right, liver toxicity index plasma alanine aminotransferase (ALT) is increased in LKO and LPKO livers indicating liver injury. n=3–5.a, different from CON; b, different from LKO; c. different from PKO. p<0.05. Data expressed as Mean±SEM.