Skip to main content
. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: NPJ Regen Med. 2017 Jan 5;2:16017. doi: 10.1038/npjregenmed.2016.17

Figure 2.

Figure 2

Cxcl14 knockdown enhances C2C12 differentiation. (a) C2C12 cells were infected with lentiviruses expressing shCxcl14 or shScramble (negative control), selected for 2 days then differentiated for 72 h, followed by staining for MHC (green) and DAPI (pseudocoloured red) (n = 3). Scale bar: 50 μm. (b) Cells treated as in (a) were differentiated and at indicated time points of differentiation (‘Hrs diff’) were lysed and subjected to western analysis (n = 3). (c) Densitometry was performed on blots in (b), and the relative levels of proteins using tubulin as reference were quantified. Paired t test was performed to compare control and shCxcl14 at each time point. *P < 0.05; **P < 0.01. (d) C2C12 cells were treated as in (a), then grown in the presence or absence of 25 ng/ml recombinant Cxcl14 (rCxcl14) for 24 h after selection. Cells were then differentiated for 3 days, followed by staining for MHC and DAPI and quantification of fusion index (n = 4). Scale bar: 50 μm. Paired two-tailed t-test was performed for the data in (d). The data denoted by different letters (a-c) are significantly different from each other (P < 0.05). All error bars represent s.d. of independent replicates.