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. Author manuscript; available in PMC: 2017 Jul 11.
Published in final edited form as: J Vis Exp. 2016 Dec 19;(118):10.3791/52965. doi: 10.3791/52965

Figure 2. Endothelial Cell Phenotypes.

Figure 2

The attached cells display spindle-shaped and cobblestone-like appearances (A, bar = 100 μm), double-positive fluorescence of Dil-ac-LDL and Ulex-Lectin (B–E, bar = 100 μm). Meanwhile, platelet endothelial cell adhesion molecule 1 (CD31, PECAM-1), VEGFR2, VE-Cadherin, eNOS were positive in >95% of the cells after the second passage (F–I, bar = 100 μm) Most of the cells were negative for Calponin staining, which is a smooth muscle cell marker (J, bar = 100 μm).