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. Author manuscript; available in PMC: 2017 Dec 20.
Published in final edited form as: J Endocr Soc. 2017 Mar 6;1(4):370–384. doi: 10.1210/js.2016-1071

Figure 5.

Figure 5

FOXA2 is the major protein in mouse islets binding the FOXA motif in the proximal ACE2 promoter. (a) An EMSA with the human R6 probe was conducted using nuclear extracts from mouse pancreatic islets and 832/13 cells. (b) An EMSA with the mouse R6 probe was conducted using cytoplasmic and nuclear extracts from mouse pancreatic islets. Antibodies against FOXA transcription factors were included in binding reactions as indicated. (c) The concentrations of FOXA2 mRNA and ACE2 mRNA relative to 18S ribosomal RNA were determined in freshly isolated pancreatic islets from six male and five female mice. We depict the ACE2 values against the FOXA2 values for each mouse. The solid and stippled lines are the regression lines for females and males, respectively. The concentrations of FOXA2 and ACE2 showed significant (P < 0.01) correlation for both males and females as indicated by the correlation coefficients R. (d) The content of ACE2 mRNA relative to FOXA2 mRNA was calculated. *P < 0.05 vs males.