Skip to main content
. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: J Vis Exp. 2016 Nov 3;(117):10.3791/55007. doi: 10.3791/55007

Figure 1. Visual summary of the zebrafish cancer cell extravasation assay.

Figure 1

In this assay, cancer cells are labeled with a fluorescent dye and injected into the precardiac sinus of 2-day old zebrafish embryos, whose vasculature is marked by a contrasting fluorescent reporter. Following 2–4 additional days, cancer cells that have invaded into the caudal region of the embryo are scored and can be mounted in an anesthetic agarose medium for subsequent imaging. Each step depicted in this schematic corresponds to a section of the protocol.