AC8 KO mice show impaired acquisition of newer spatial information. After the hidden platform trials, WT and AC8 KO mice were trained by the reversal test, during which the platform was moved from quadrant 2 to 4 (as indicated by the inset diagram) (A). After 4 days of training, both groups (WT and AC8 KO mice) learned the reversed platform position, as indicated by the improvement in escape latency (A). A probe trial was carried out after the reversal protocol. Both WT and AC8 KO mice spent significant more time in the new target quadrant (quadrant 4) (B). After the reversal paradigm, the mice were further trained to find 2 new platform positions (placed in quadrant 3 and 2) on 2 consecutive days (C). All values are expressed as average +/− SEM.