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AC8 is co-purified with Rab3A in the presynaptic active zone (PAZ). Brain homogenates from WT, AC1 KO, and AC8 KO mice were separated into extrasynaptic, PAZ and PSD fractions. The presence of AC1 (A), AC8 (B), PSD-95 (C), and Rab3A (D) was detected by Western blot analysis.
