Fig. 2.

Vaccination with AMA1-RON2L complex induces a shift in the quality of blocking antibody. a FVO AMA1-specific antibody levels in plasma and purified IgG. ELISA was performed on individual samples collected before the day of challenge. Arbitrary ELISA units based on standard curves were generated to compare anti-FVO AMA1 antibody levels in plasma and purified IgG of Group 2 and Group 3 animals by the Mann–Whitney test (plasma: P = 0.854; IgG: P = 0.045). Data are shown for individual animals and represented as mean ± SEM. (b) In vitro GIA using purified IgG against the homologous FVO strain. Total IgG from each animal was tested at 2.5 mg mL⁻¹ final concentration and inhibitory activity was compared between Group 2 and 3 by Mann–Whitney test (P = 0.0006). Data shown are from two independent experiments represented as mean ± SEM. Antibody data are from plasma samples collected 4 weeks after last vaccination (before parasite challenge)