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. Author manuscript; available in PMC: 2017 Jul 26.
Published in final edited form as: Bio Protoc. 2017 May 20;7(10):e2281. doi: 10.21769/BioProtoc.2281

Figure 3. Fragments of PCR products and pSPL3 vector following restriction digestion with BamHI and XhoI endonucleases.

Figure 3

The TEK exon 5 (E5) and exon 22 (E22) fragments are observed at 1,463 bp and 837 bp, respectively. The pSPL3 vector (V) fragment is observed at 6,011 bp. A sample of undigested supercoiled pSPL3 vector (UV) is also included on the gel, migrating at ~4,000 bp. 1% agarose gel; molecular weight markers are 5 μl of ‘HyperLadder 1 kb’ per lane.