| cDNA template | 4 μl |
| 10x HotStarTaq buffer | 4 μl |
| 2 mM dNTPs | 4 μl |
| 10 μM V1-F primer | 2 μl |
| 10 μM V2-R primer | 2 μl |
| HotStarTaq polymerase (5 U/μl) | 0.4μl |
| Nuclease-free water | 23.6 μl |
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