| Initial denaturation | 95 °C | 5 min |
| 35 cycles of | 94 °C | 30 sec (denaturation) |
| 55 °C | 30 sec (annealing temperature of primers) | |
| 72 °C | 3 min (30 sec per kilobase extension) | |
| Final extension | 72 °C | 10 min |
| Hold temperature | 4 °C |
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