Skip to main content
. Author manuscript; available in PMC: 2018 Feb 9.
Published in final edited form as: J Vis Exp. 2017 Feb 9;(120):10.3791/55183. doi: 10.3791/55183

Figure 4. Microvascular networks in the rat mesentery culture model were imaged before and after angiogenesis.

Figure 4

Comparison of the same network labeled with lectin on day 0 and day 3 (A, B) post-stimulation with 10 % serum identifies new vessels. Lectin also labels a population of unidentified interstitial cells. Quantification of vessel density (C, D) and the number of capillary sprouts per vascular area (E, F) confirmed an increase in both metrics for each tissue. C, E) Before (day 0) and after (day 3) comparisons per tissue. D, F) Comparison between day 0 and day 3 averages using a paired Student’s t-test confirmed a significant difference in both the average number of vessel segments (p < 0.0001) and the average number of sprouts (p < 0.00001) per vascular area. White bars represent day 0, and black bars represent day 3. Values are averages ± SEM. For this representative analysis, 13 tissues were harvested from 2 rats. Scale bars = 100 μm.