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. Author manuscript; available in PMC: 2018 Feb 9.
Published in final edited form as: J Vis Exp. 2017 Feb 9;(120):10.3791/55183. doi: 10.3791/55183

Figure 5. The rat mesentery culture model can be used for investigating vascular island fate and incorporation into nearby networks.

Figure 5

Using time-lapse imaging, vascular islands were identified on day 0 and their connection to the nearby network was confirmed by day 3 post-angiogenic stimulation. Mesentery tissues were stimulated with bFGF (A, B) and VEGF/PDFG-BB (C, D). Hollow arrows show disconnected segments on day 0 and solid arrows represent island connection to the network. Arrowheads indicate the location of connections between a vascular island and the nearby network. Scale bars = 100 μm.