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. Author manuscript; available in PMC: 2018 Feb 9.
Published in final edited form as: J Vis Exp. 2017 Feb 9;(120):10.3791/55183. doi: 10.3791/55183

Table.

Table of Specific Reagents

Name Company Catalog Number Comments/Description
Beuthanasia Schering-Plough Animal Health Corp. Union (Ordered from MWI Veterinary Supply) MWI #: 011168 Active Ingredient: Per 100mL, 390 mg pentobarbital sodium, 50mg phenytoin sodium
Ketamine Fort Dodge Animal Health (Ordered from MWI Veterinary Supply) MWI #: 000680 Kateset 100 mg/ml
Xylazine LLOYD. Inc. (Ordered from MWI Veterinary Supply) MWI #: 000680 Anased 100 mg/ml
Saline Baxter 2F7122
PBS Invitrogen 14040-133
MEM Invitrogen 11095080
PenStrep Invitrogen 15140-122
FBS Invitrogen 16000-044
BSA Jackson ImmunoResearch 001-000-162
Saponin SIGMA S7900-100G
Isopropyl Alcohol Fisher Scientific S25372
Povidone-Iodine Operand 82-226
Hydrochloric Acid SIGMA 320331
Methanol Fisher Scientific 67-56-1
Glycerol Fisher Scientific 56-81-5
FITC-conjugated Lectin SIGMA L9381-2MG
Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody SIGMA AB5320
PECAM (CD31) Antibody BD Biosciences 555026
LYVE-1 Antibody AngioBio Co. 11-034
Goat Anti-Rabbit Cy2-conjugated Antibody Jackson ImmunoResearch 111-585-144
Goat Anti-Mouse Cy3-conjugated Antibody Jackson ImmunoResearch 115-227-003
Streptavidin Cy3-conjugated Antibody Jackson ImmunoResearch 016-160-084
Live/Dead Viability/Cytotoxicity Kit Invitrogen L3224
Normal Goat Serum Jackson ImmunoResearch 005-000-121
5-Bromo-2’-Deoxyuridine SIGMA B5002
Monoclonal Mouse Anti-Bromodeoxyuridine
Clone Bu20a Dako M074401-8
Mouse Anti-Rat CD11b AbD Serotec MCA275R
Drape Cardinal Health 4012 12"×12" Bio-Shield Regular Sterilization Wraps
Scalpel Handle Roboz Surgical Instrument RS-9843 Scalpel Handle, #3; Solid; 4" Length
Sterile Surgical Blade Cincinnati Surgical 0110 Stainless Steel; Size 10
Culture Dish (60mm) Thermo Scientific 130181 10/Sleeve
Graefe Forcep (curved tweezers) Roboz Surgical Instrument RS-5135 Micro Dissecting Forceps; Serrated; Slight Curve; 0.8mm Tip Width; 4" Length
Graefe Forcep (straight tweezers) Roboz Surgical Instrument RS-5130 Micro Dissecting Forceps; Serrated, Straight; 0.8mm Tip Width; 4" Length
Noyes Micro Scissor Roboz Surgical Instrument RS-5677 Noyes Micro Dissecting Spring Scissors; Straight, Sharp-Blunt Points; 13mm Cutting Edge; 0.25mm Tip Width, 4 1/2" Overall Length
Gauze Pads FisherBrand 13-761-52 Non-Sterile Cotton Gauze Sponges; 4"x4" 12-Ply
Cotton-Tippled Applicators FisherBrand 23-400-124 6" Length; Wooden Shaft; Single Use Only
6-Well Plate Fisher Scientific 08-772-49 Flat Bottom with Low Evaporation Lid; Polystyrene; Non-Pyrogenic
Sterile Syring 5ml Fisher Scientific 14-829-45 Luer-Lok Tip
Sterile Bowl Medical Action Industries Inc. 01232 32 oz. Peel Pouch; Blue; Sterile Single Use
6-Well Plate Inserts (CellCrown Inserts) SIGMA Z681792-3EA 6-Well Plate Inserts; Non-Sterile
Polycarbonate Filter Membrane SIGMA TMTP04700 Isopore Membrane Filter; Polycarbonate; Hydrophilic; 5.0 μm, 47 mm, White Plain