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. Author manuscript; available in PMC: 2017 Aug 3.
Published in final edited form as: J Dev Biol. 2016 Sep 29;4(4):29. doi: 10.3390/jdb4040029

Figure 6.

Figure 6

Ectopic expression of Ptch1 in the E16.5 hGFAPcre/+;Sufufl/fl VZ/SVZ. (A–C) quantitative PCR (qPCR) analysis of mRNA extracted from the neocortex showed downregulation of Sufu as expected in the E16.5 hGFAPcre/+;Sufufl/fl neocortex (A); No changes in Gli1 expression were detected between control and mutant neocortex as previously reported (B); However, a significant increase in the expression of Ptch1 was detected in the E16.5 hGFAPcre/+;Sufufl/fl neocortex compared to controls (C) indicating activation of Sonic Hedgehog (Shh) signaling; *** p-value < 0.01; (D) In situ hybridization using Patched-1 (Ptch1)-specific riboprobes showed ectopic expression of Ptch1 in the VZ/SVZ of the E16.5 hGFAPcre/+;Sufufl/fl neocortex. Scale bars = 200 μm.